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universal bacterial 16s rrna primers  (ATCC)


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    ATCC universal bacterial 16s rrna primers
    Universal Bacterial 16s Rrna Primers, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6358 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/universal+bacterial+16s+rrna+primers/16S/10__7554_slash_elife__92994-355-16-25
    Average 99 stars, based on 6358 article reviews
    universal bacterial 16s rrna primers - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Peptidoglycan-Chi3l1 interaction shapes gut microbiota in intestinal mucus layer
    Article Snippet: A PCR reaction was performed using 2 μL of the bacterial suspension as template DNA and universal bacterial 16S rRNA primers (27F, 5’- AGAG TTTG ATCC TGGC TCAG -3’ and 1492R, 5’- GGTT ACCT TGTT ACGA CTT-3’) with reaction conditions: 95°C for 5 min followed by 35 cycles of 95°C for 30 s, 55°C for 30 s, 72°C for 2 min and then 72°C for 20 min. .. A PCR reaction was performed using 2 μL of the bacterial suspension as template DNA and universal bacterial 16S rRNA primers (27F, 5’- AGAG TTTG ATCC TGGC TCAG -3’ and 1492R, 5’- GGTT ACCT TGTT ACGA CTT-3’) with reaction conditions: 95°C for 5 min followed by 35 cycles of 95°C for 30 s, 55°C for 30 s, 72°C for 2 min and then 72°C for 20 min. ..

    Suspension:

    Article Title: Peptidoglycan-Chi3l1 interaction shapes gut microbiota in intestinal mucus layer
    Article Snippet: A PCR reaction was performed using 2 μL of the bacterial suspension as template DNA and universal bacterial 16S rRNA primers (27F, 5’- AGAG TTTG ATCC TGGC TCAG -3’ and 1492R, 5’- GGTT ACCT TGTT ACGA CTT-3’) with reaction conditions: 95°C for 5 min followed by 35 cycles of 95°C for 30 s, 55°C for 30 s, 72°C for 2 min and then 72°C for 20 min. .. A PCR reaction was performed using 2 μL of the bacterial suspension as template DNA and universal bacterial 16S rRNA primers (27F, 5’- AGAG TTTG ATCC TGGC TCAG -3’ and 1492R, 5’- GGTT ACCT TGTT ACGA CTT-3’) with reaction conditions: 95°C for 5 min followed by 35 cycles of 95°C for 30 s, 55°C for 30 s, 72°C for 2 min and then 72°C for 20 min. ..



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    Phylogenetic tree based on bacterial <t>16S</t> <t>rRNA</t> genes. Lane M: DL-2000 marker; Lane 1: negative control; Lanes 2–5: 16S rRNA amplification results of isolated strains.
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    Image Search Results


    Neighbor-joining phylogenetic tree of strictly anaerobic and culturable bacteria isolated from the feces of T. molitor larvae based on 16S rRNA gene sequences; the effective sequence length was ~1,500 bp. This tree was constructed using the MEGA11 software package. Bar, 0.01 evolutionary distances. Sequences labeled with an orange color represent novel species or genera.

    Journal: Frontiers in Microbiology

    Article Title: Diversity and community composition of strictly anaerobic and culturable bacteria from the feces of Styrofoam-fed Tenebrio molitor larvae: a culturomics-based study

    doi: 10.3389/fmicb.2023.1309806

    Figure Lengend Snippet: Neighbor-joining phylogenetic tree of strictly anaerobic and culturable bacteria isolated from the feces of T. molitor larvae based on 16S rRNA gene sequences; the effective sequence length was ~1,500 bp. This tree was constructed using the MEGA11 software package. Bar, 0.01 evolutionary distances. Sequences labeled with an orange color represent novel species or genera.

    Article Snippet: The 16S rRNA gene fragment of each purified isolate was amplified using bacterial 16S rRNA gene universal primer set of 8F (5’-AGAGTTTGATCCTGGCTCAG-3′) ( ) and 1492RU (5’-TTTTAATTAAGGTTACCTTGTTACGACTT-3′) , which were ordered from Sangon Biotechnology (Shanghai) Co., Ltd.

    Techniques: Bacteria, Isolation, Sequencing, Construct, Software, Labeling

    Results are shown for a 4-plex assay (format used for analysis of 192 B. napus DNA extracts) on plasmid DNA controls (10 7 copies/PCR) and on genomic DNA extracted from various infected plant tissues. Beads with a positive hybridization signal in each sample are identified (*). Samples from infected plant tissues are those described in (onion, item #25; flax, item #41; vinca1, item #33; vinca2, item #35; carrot, item #15). Abbreviations: MFI, median fluorescence intensity; BN, Bois Noir; ESFY, European Stone Fruit Yellows; AY, Aster Yellows.

    Journal: PLoS ONE

    Article Title: Molecular Diagnostic Tools for Detection and Differentiation of Phytoplasmas Based on Chaperonin-60 Reveal Differences in Host Plant Infection Patterns

    doi: 10.1371/journal.pone.0116039

    Figure Lengend Snippet: Results are shown for a 4-plex assay (format used for analysis of 192 B. napus DNA extracts) on plasmid DNA controls (10 7 copies/PCR) and on genomic DNA extracted from various infected plant tissues. Beads with a positive hybridization signal in each sample are identified (*). Samples from infected plant tissues are those described in (onion, item #25; flax, item #41; vinca1, item #33; vinca2, item #35; carrot, item #15). Abbreviations: MFI, median fluorescence intensity; BN, Bois Noir; ESFY, European Stone Fruit Yellows; AY, Aster Yellows.

    Article Snippet: Samples were analyzed using 16S rRNA gene-targeted universal bacterial PCR primers to ensure that amplifiable bacterial DNA was present in the extract at the dilutions analyzed. cpn 60 UT amplicons were generated under the following conditions: 1×PCR buffer (Life Technologies); 2.5 mM MgCl 2 ; 0.4 µM each primer ( ); 0.5 mM each dNTP; and 1 U Taq DNA polymerase (Life Technologies).

    Techniques: Plex Assay, Plasmid Preparation, Infection, Hybridization, Fluorescence

    Phylogenetic tree based on bacterial 16S rRNA genes. Lane M: DL-2000 marker; Lane 1: negative control; Lanes 2–5: 16S rRNA amplification results of isolated strains.

    Journal: Veterinary Sciences

    Article Title: Rothia nasimurium as a Cause of Disease: First Isolation from Farmed Chickens

    doi: 10.3390/vetsci9120653

    Figure Lengend Snippet: Phylogenetic tree based on bacterial 16S rRNA genes. Lane M: DL-2000 marker; Lane 1: negative control; Lanes 2–5: 16S rRNA amplification results of isolated strains.

    Article Snippet: The universal primers for bacterial 16S rRNA were synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd. (Sangon Biotech, Shanghai, China).

    Techniques: Marker, Negative Control, Amplification, Isolation